Suppression of SUS alters the expression patterns of genes involved in auxin signaling and leaf morphology. Error bars indicate standard error ( n = 5). (A) Sucrose, glucose and fructose contents of young green fruit. SlSUS suppression does not significantly affect soluble sugars or starch content. Note the angle of the petiolules (arrowheads) and of the leaflet curling (arrow). (A) Mature S1R4 leaf, (B) mature WT leaf, (C) magnification of the boxed areas in the S1R4 leaf, (D) magnification of the boxed areas in the WT leaf and (E) close-ups of the adaxial and (F) the abaxial side of an S1R4 leaf, showing ectopic blade formation of the leaflets (red area). The S1R4 line exhibits abnormal leaf morphology. Asterisks indicate a statistically significant difference relative to the WT (* P < 0.05 ** P < 0.01). (C) To calculate average seed weights, 50 seeds per fruit ( n = 4) were counted and weighed, and that weight was divided by the number of seeds. (B) Fruit set was calculated as the number of fruit divided by the number of flowers from the first five inflorescences of each plant ( n = 9). Flowers from the first five inflorescences from the ground of each plant ( n = 9) were counted. (A) SlSUS-RNAi and WT plants were grown in a greenhouse to maturity. Asterisks indicate a statistically significant difference from the WT (* P < 0.05 ** P < 0.01).Įffect of SlSUS suppression on tomato fertility and seed viability. Error bars indicate the standard error ( n ≥ 3). Cyclophilin was used as a reference gene. cDNA was generated and subjected to real-time PCR analysis, using primers specific for SlSUS1, 3 and 4. RNA was extracted from green fruits, shoot apices, and mature leaves of SlSUS-RNAi and WT lines. SlSUS-RNAi lines exhibit SlSUS co-suppression. The warped cotyledon of the S1R4 embryo is clearly visible bar– 0.5 mm. (B) S1R4 embryo and (C) WT embryo extracted from seeds soaked for 24 h. Each pair of cotyledons was taken from a single seedling. (A) Cotyledons from wild-type (WT) seedlings and seedlings of three SlSUS-RNAi lines. SlSUS-RNAi lines exhibit abnormal cotyledon morphology. Leftmost column: cross-sections of ungerminated seeds center column: radicle tips of 3-day-old seedlings rightmost column: seedling roots, showing the initiation of root branches (arrowheads) bar– 0.5 mm. Leftmost column: flowers before anthesis second column from left: flowers at anthesis third column from left: ~1 cm green fruit rightmost column: ripe fruit bar– 2 mm.Įxpression patterns of the three SlSUS genes in embryos and seedling roots.Ĭomparative GUS stains of germinating seeds and seedling roots from tomato lines expressing GUS under the control of each of the three SlSUS promoters ( proSlSUS). Leftmost column: whole stem segment, bar– 1 mm second column from left: magnifications of leftmost panels, showing axillary shoots (arrows), bar– 0.5 mm third column from left: micrographs of stem cross-sections, bar– 0.5 mm rightmost column: magnification of boxed areas from previous panels EP–external phloem IP–internal phloem XY–xylem vessel members bar– 0.1 mm.Įxpression patterns of the three SlSUS genes in reproductive organs.Ĭomparative GUS stains of flowers and fruit from tomato lines expressing GUS under the control of each of the three SlSUS promoters ( proSlSUS). RPKM, reads per kilobase per millions mapped reads.Įxpression patterns of the three SlSUS genes in young stems.Ĭomparative GUS stains of stems from tomato lines expressing GUS under the control of each of the three SlSUS promoters ( proSlSUS). SlSUS gene family expression data obtained from RNA seq in different organs of the M82 cultivar. SlSUS genes relative expression in different organs. Characteristics of sucrose synthase genes in tomato.
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